mitf (OriGene)
Structured Review

Mitf, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitf+rabbit+polyclonal+antibody/MITF+Rabbit+Polyclonal+Antibody/pmc08533235-54-28-29
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "RICTOR Affects Melanoma Tumorigenesis and Its Resistance to Targeted Therapy"
Article Title: RICTOR Affects Melanoma Tumorigenesis and Its Resistance to Targeted Therapy
Journal: Biomedicines
doi: 10.3390/biomedicines9101498
Figure Legend Snippet: RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated STAT3 (Y705), total STAT3, MITF, SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).
Techniques Used: Western Blot, Transfection, Plasmid Preparation, Control, Expressing
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